METHOD FOR PREPARING PACLITAXEL OF HIGH YIELD BY SUSPENSION CULTURE OF TAXUS WALLICHIANA ZUCC. STEM CELLS

摘要

PURPOSE: A method for preparing paclitaxel and Taxus wallichiana Zucc. stem cells is provided to promote cell growth and to enhance paclitaxel yield.;CONSTITUTION: A method for preparing Taxus wallichiana Zucc. stem cells and preparing paclitaxel comprises: a step of inducing callus from an alive tissue of Taxus wallichiana Zucc. in a Murashige & Skoogs solid medium for inducing callus; and a step of suspension culture. The method also comprises a step of administering jasmonic acid and cellulase.;COPYRIGHT KIPO 2012