Method of determination of nucleotides in foods by means of liquid chromatography of ionic pairs with detection by photodiodos coupled to spectrometry of masses. (Machine-translation by Google Translate, not legally binding)

摘要

Method of determination of nucleotides in food by ion pair liquid chromatography with photodiode detection coupled to time-of-flight mass spectrometry with dual ionization operating in negative mode (hplc/esi-apci-tof-ms). The method allows rapid determination of the five authorized monophosphate nucleotides in infant foods (cytidine 5'-monophosphate, uridine 5'-monophosphate, adenosine 5'-monophosphate, inosine 5'-monophosphate and guanosine 5'-monophosphate), and comprises pre-precipitation of the food proteins and direct analysis of the nucleotides in the resulting extract by hplc of ion pairs using isocratic elution. The recovery obtained for fortified samples was satisfactory for all analytes. The proposed procedure allows the determination of the only authorized nucleotides and has been applied successfully to the analysis of different samples of infant and functional foods, such as purees, cereals and baby food. (Machine-translation by Google Translate, not legally binding)