LIGHT-PAD MICROSCOPE FOR SPATIALLY RESOLVED FLUORESCENCE CORRELATION SPECTROSCOPY

摘要

The present disclosure teaches a microscope (1) having an illumination light path (20) for illuminating a sample or object (18) and a viewing light path (40, 50) for viewing the sample. The microscope comprises an illumination light path focussing arrangement in the illumination light path, the illumination light path focussing arrangement defining a substantially two- dimensional sample or object illumination region (22) extending along an illumination direction of the illumination light path and transversely thereto. The microscope further comprises an illumination region - confining device (27) in the illumination light path for selectively illuminating a portion (10) of the substantially two-dimensional object illumination region, wherein the portion of the substantially two-dimensional object illumination region is confined at least in the illumination direction and/or in the direction transversely thereto.