A METHOD FOR THE PRODUCTION OF HYBRIDOMA CELL LINES PRODUCING MONOCLONAL ANTIBODIES CAPABLE TO SPECIFICALLY BINDING TO A HUMAN C44-FRAGMENT OF AGRIN

摘要

A method for the production of a hybridoma cell lines producing monoclonal antibodies capable to specifically binding to a human C44-fragment of agrin, comprising administering to wild-type-mice an immunizing amount of C44y≥4- fragment of agrin, isolating antibody producing cells from the immunized mice, fusing them with a myeloma cell line, growing the fused cells in a selection medium, screening the antibodies in the supernatants of hybridoma cells for binding to C44- fragment of agrin and isolating the hybridoma cells producing the desired monoclonal antibodies