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A METHOD FOR THE PRODUCTION OF HYBRIDOMA CELL LINES PRODUCING MONOCLONAL ANTIBODIES CAPABLE TO SPECIFICALLY BINDING TO A HUMAN C44-FRAGMENT OF AGRIN
A METHOD FOR THE PRODUCTION OF HYBRIDOMA CELL LINES PRODUCING MONOCLONAL ANTIBODIES CAPABLE TO SPECIFICALLY BINDING TO A HUMAN C44-FRAGMENT OF AGRIN
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机译:一种生产能够特异性结合人AG44片段的单克隆抗体的杂交细胞系的生产方法
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摘要
A method for the production of a hybridoma cell lines producing monoclonal antibodies capable to specifically binding to a human C44-fragment of agrin, comprising administering to wild-type-mice an immunizing amount of C44y‰¥4-fragment of agrin, isolating antibody producing cells from the immunized mice, fusing them with a myeloma cell line, growing the fused cells in a selection medium, screening the antibodies in the supernatants of hybridoma cells for binding to C44-fragment of agrin and isolating the hybridoma cells producing the desired monoclonal antibodies
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