GENETIC ENGINEERING STRAIN FOR PRODUCING SUCCINIC ACID BY USING GLUCOSE AND METHOD FOR PRODUCING ACID BY FERMENTING THE STRAIN

摘要

A genetic engineering strain for producing succinic acid by using glucose and a method for producing acid by fermenting the strain are provided. The genetic engineering strain for producing succinic acid is E.coli strain BA205, deposited as CCTCC NO: M2011447. The construction process comprises: using the E.Coli strain lacking activities of lactate dehydrogenase (LDH) gene and pyruvate formate lyase (PFL) gene as the original strain, knockouting phosphoenolpyruvate carboxylase (PPC) gene by using homologous recombination technology, and over co-expressing phosphoenolpyruvate carboxylated kinase gene and nicotinic acid phosphoribosyl transferase. The method for producing succinic acid using the genetic engineering strain employs two-stage fermentation. With the biomass being increased in aerobic stage, and with acid being produced by fermentation in anaerobic stage.