A NOVEL FABRICATED TRANSLATIONAL FUSION PARTNER FOR SECRETORY PRODUCTION OF FOREIGN PROTEIN, A PROCESS FOR PREPARING THE FOREIGN PROTEIN USING THE SAME AND A SCREENING METHOD OF THE SAME

摘要

PURPOSE: An artificial protein translational fusion partner comprising the combination of pre-secretion signal and pro-secretion signal for producing and secreting foreign protein is provided to mass-produce foreign protein hardly mass-produced in yeast due to a traditional recombinant technique with hGH. CONSTITUTION: An artificial protein translational fusion partner for producing and secreting foreign protein comprises the pre-secreting signal and pro-secretion signal of protein translational fusion partner from yeast. The pre-secretion signal and pro-secretion signal are picked from different protein translational fusion partners. Pre-secretion signal is the protein in the sequence number from 1 (SEQ ID NO:1) to 25 (SEQ ID NO:25) the sequence number from 51 (SEQ ID NO:51) to 75 (SEQ ID NO:75. Pro-secretion signal is the protein chosen in the sequence number from 1 (SEQ ID NO:1) to 25 (SEQ ID NO:25 or the gene chosen in the sequence number from 51 (SEQ ID NO:51) to 75(SEQ ID NO:75). The foreign protein is human growth hormone and pre-secretion signal is TFP 20-pre(the sequence number 14 and 64) and pro-secretion signal is TFP3-pre(the sequence number 3 and 53) or TFP 3-pre(the sequence number 10 and 60). A method of producing foreign protein comprises the following steps. An expression vector with an artificial protein translational fusion factor and a foreign protein gene to be produced is made(i). The expression vector in step (i) is introduced in yeast so that a transformant is produced(ii). The transformant is cultivated and foreign protein is obtained in a culture or a supernatant layer(iii).