BACTERIAL FIXATION, DRUG DIFFUSION, AND SINGLE BACTERIAL CELL TRACKING USING A SOLIDIFYING AGENT, AND A QUICK DRUG TEST SYSTEM BASED ON THE SAME

摘要

PURPOSE: A test method using fixation of a biological substance and diffusion of a physiologically active substance is provided to largely reduce the amount of a drug and cells for a drug test, and to quickly trace single cell growth.;CONSTITUTION: A physiological activity test method is performed by observing response of a biological substance with respect to a physiologically active substance. The method comprises the steps of: providing a mixture solution of the biological substance and a liquid medium containing a solidifying agent to a substrate for thin film formation (S1); solidifying the mixture solution and forming a biological substance-fixed solid thin film (S2); supplying the physiologically active substance to the solid thin film to diffuse the physiologically active substance into the solid thin film (S3); and determining minimum inhibitory concentration (MIC) of the physiologically active substance based on a result obtained by imaging individual response of single cells of the biological substance with respect to the physiologically active substance.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Start; (BB) End; (S1) Provide a mixture solution of a biological substance and a liquid medium containing a solidifying agent to a substrate; (S2) Form a biological substance-fixed solid thin film by solidifying the mixture solution; (S3) Diffuse a physiologically active substance into the solid thin film; (S4) Observe the reaction of the biological substance with respect to the physiologically active substance