PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF INACTIVE PRECURSOR OF MUTEIN C112S OF HUMAN ENTEROKINASE LIGHT CHAIN, BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT MUTEIN C112S OF HUMAN ENTEROKINASE LIGHT CHAIN, PRECURSOR OF RECOMBINANT MUTEIN C112S OF HUMAN ENTEROKINASE LIGHT CHAIN, METHOD OF PRODUCTION OF RECOMBINANT MUTEIN C112S OF HUMAN ENTEROKINASE LIGHT CHAIN, RECOMBINANT MUTEIN C112S OF HUMAN ENTEROKINASE LIGHT CHAIN

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, and represents a method of producing a recombinant mutein [C112S] of human enterokinase light chain, using a bacterium belonging to the genus Escherichia, transformed with an expression plasmid containing a DNA fragment encoding a mutein [C112S] inactive precursor of human enterokinase light chain, comprising a sequence encoding a cleavable N-terminal peptide comprising a hexahistidine cluster and the enterokinase recognition sequence, and a sequence fused with it in frame encoding a precursor of mutein [C112S] of human enterokinase light chain with non-cleavable C-terminal hexahistidine cluster under control of a promoter operating in the bacterial cell.EFFECT: invention enables to produce the recombinant mutein of human enterokinase light chain with a high yield.11 cl, 5 dwg, 1 tbl, 6 ex