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The cell culture manner which is due to the intercellular signal conditioning which uses powdered foothold
The cell culture manner which is due to the intercellular signal conditioning which uses powdered foothold
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机译:细胞培养方式是由于使用粉状立足点的细胞间信号调节
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摘要
A three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells is provided. The method includes repeatedly performing any one or both of the following processes a) and b) so as to regulate proliferation-inducing and proliferation-inhibitory signals between the cells: a) a process of gradually adding the micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage in order to maintain a suitable distance between the cells, and the amount of the micro-scaffolds is then increased slowly according to cell proliferation rate; and b) a periodic shaking process, in which shaking is performed in order to separate connected cells from each other by a physical force, after the cells are incubated for more than a given period of time.
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