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PURIFICATION OF NON-HUMAN ANTIBODIES USING KOSMOTROPIC SALT ENHANCED PROTEIN A AFFINITY CHROMATOGRAPHY
PURIFICATION OF NON-HUMAN ANTIBODIES USING KOSMOTROPIC SALT ENHANCED PROTEIN A AFFINITY CHROMATOGRAPHY
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机译:利用亲和盐增强蛋白纯化亲和层析法纯化非人类抗体
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摘要
The present invention is directed to methods for purifying a non-human antibody, or antigen binding portion thereof, exhibiting weak binding strength and low binding capacity for Protein A chromatography media. In one aspect, a kosmotropic salt solution is employed to promote the hydrophobic interaction between the non-human antibody, or antigen binding portion thereof, and the Protein A ligand, thereby enhancing the binding of the non-human antibody, or antigen binding portion thereof, to the Protein A chromatography media. In another aspect, the concentration of the non-human antibody, or antigen binding portion thereof, in a sample comprising the antibody, or antigen binding portion thereof, exposed to a Protein A chromatography media is increased to enhance the binding of the non-human antibody, or antigen binding portion thereof, on the Protein A chromatography media.
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