Optimization of protein purification in chromatography processes: Selection of optimum affinity tags from a phage display library and mathematical modeling with immobilized metal affinity chromatography as a case study.

摘要

Immobilized Metal Affinity Chromatography (IMAC) has shown promise for protein separation based on differential afinity of proteins for chelated metal ions. IMAC used for the purification of proteins with insignificant metal affinity, by their fusion to metal binding peptides as affinity tails. A tail consisting of six adjacent histidines (His