DESIGNING OF AN NOVEL OPTICAL BIOSENSOR FOR DETECTION OF ORGANOPHOSPHORUS PESTICIDES

摘要

The present investigation deals with novel Design of an optical Biosensor for detection of organophosphorus pesticides. We have designed an optical based disposable microbial biosensor to determine methyl parathion pesticide. The sensing scheme was based on the measurement of product formation p-nitrophenol (PNP) by Organophosphorus hydrolase due to the hydrolysis of methyl parathion pesticide. The detection with optics biosensor is a simple, single step and direct Flavobacterium sp. NCIM 5107 microbial glass fiber disc was disposable. Disposable biocomponent was cost effective for methyl parathion detection. The absorbance of p-nitrophenol was detected at 401nm on UV-Visible spectrophotometer, by the proposed optics biosensor system. The designing of optics biosensor was done in three major steps - A) Design of reaction vessel, B) Design of assembly of biosensor and C) circuit design of biosensor. The sensing scheme leads to a direct determination of analyte as the rate of signal generation is directly proportional to the concentration of organophosphate. This biosensor is can be used as an important tool by various Environment Pollution Monitoring and Screening Agencies(Pollution Control Board) that have to keep a check on the increasing pollution and take preventive measures to curb them. This will ultimately result in the welfare of mankind and prove beneficial for the society.