Method for producing of alginate coatings on alginate spheres chamber and a method for protecting living cells encapsulated in these areas or regulate the release of biologically active substances immobilized/encapsulated in these areas

摘要

method of manufacturing otoczek alginianowych on ventricular alginianowych spheres for enkapsulacji living cells and chemical restraint / enkapsulacji biologically active substances.is that alginianowe spheres containing ca + 2 ions ventricular or ba + 2introduced in the process of their manufacture or by immersion these spheres in solution calcium chloride or barium chloride, is placed in the solution of sodium alginate,whether or not containing sodium chloride, and then transferring them to the water or the aqueous solution of sodium chloride, intensively mixedalternatively, subsequently the ventricular immersed in solution of calcium chloride or barium chloride. the procedure described above is repeated at least once or not.way of safeguarding living cells enkapsulowanych in alginianowych spheres ventricular or regulate release active substances biologically immobilizowanych / enkapsulowanych in those areas.is that alginianowe the ventricular, containing enkapsulowane live cells or immobilizowane / enkapsulowane biologically active substances.in addition, ions containing ca + 2 or ba + 2 introduced in the manufacturing process or as a result of these spheres of volumetric immersion spheres in solution calcium chloride or barium chloride.placed in the solution of sodium alginate, optionally containing sodium chloride, and then transferring them to water or to an aqueous solution of sodium chlorideafter any immersed in solution of calcium chloride or barium chloride.the above procedure is repeated at least once and possibly beneficial spheres immersed in a solution of synthetic polymer and an organic solvent.preferably in solution in acetone or methyl polimetakrylanu natryskuje this solution and washed in water.the immersion in solution or spraying the solution or synthetic polymer is repeated at least once.