METHOD OF ETHANOL PRODUCTION USING SWEET SORGHUM GRAINS

摘要

THE PRESENT INVENTION RELATES TO A METHOD FOR PRODUCING ETHANOL FROM SWEET SORGHUM GRAINS, COMPRISING THE STEPS OF: MIXING SWEET SORGHUM POWDER WITH WATER AT 25-35% BY WEIGHT TO FORM A MIXTURE, WHEREBY THE PARTICLE SIZE OF THE SWEET SORGHUM POWDER IS 9-13 µM; THEN, ADDING α-AMYLASE 0.1- 0.2% BY VOLUME, RELATIVE TO THE WEIGHT OF SWEET SORGHUM POWDER, TO FORM A LIQUEFACTION MIXTURE, WHEREBY THE ENZYME ACTIVITY OF THE α-AMYLASE IS 25,000 U/mL; THEN, INCUBATING THE LIQUEFACTION MIXTURE AT 80-90° CELSIUS FOR 1 TO 2 HOURS FOR THE REACTION OF α-AMYLASE; THEN, ADDING GLUCOAMYLASE 0.1 TO 0.2% BY VOLUME, RELATIVE TO THE WEIGHT OF SWEET SORGHUM POWDER, TO FORM A SACCHARIFICATION MIXTURE, WHEREBY THE ENZYME ACTIVITY IS 130,000 U/mL; THEN, INCUBATING THE SACCHARIFICATION MIXTURE AT 45 TO 75°CELCIUS FOR TWO TO FOUR HOURS TO PRODUCE STARCH HYDROLYSATE; THEN, MIXING 0.3-0.6% UREA AND 0.05-0.10% NITROGEN-PHOSPHOROUS-POTASSIUM FERTILIZER TO THE STARCH HYDROLYSATE TO FORM A NUTRIENT MEDIA SUITABLE FOR YEAST GROWTH; THEN, ADDING APPROXIMATELY 10% BY VOLUME OF A SACCHAROMYCES CEREVISIAE INOCULUM INTO THE STARCH HYDROLYSATE; THEN, ADJUSTING THE MIXTURE PH TO PH 5 TO 7 FOR OPTIMUM CONDITION OF SACCHAROMYCES CEREVISIAE; THEN, FERMENTING THE STARCH HYDROLYSATE AT 30-35°CELSIUS WITH 60-100RPM AGITATION FOR APPROXIMATELY 72 HOURS TO PRODUCE ETHANOL.