How to select the secondary neurospheres derived from differentiated cells derived from pluripotent stem cells , clones were selected by the selection method , and how to use the clone .

摘要

In order to provide by tumor formation risk is low or in in vivo, to establish efficient iPS-derived neural stem cells without risk, side effects or less, there is no side effects, nerve damage treatment agent containing the iPS-derived neural stem cells , A clone was selected to form neurospheres via Embryoid Body from iPS cells, are activated Nanog gene promoter in cells of less than about 0.01% in secondary neurospheres, nerve damage, and clones are selected is administered to a patient having. [Selection Figure 6