Selection method of secondary neurospheres differentiated cell-derived pluripotent stem cell-derived clones, and how to use the clone that has been selected by the selection method

摘要

In order to provide by tumor formation risk is low or in in vivo, to establish efficient iPS-derived neural stem cells without risk, side effects or less, there is no side effects, nerve damage treatment agent containing the iPS-derived neural stem cells , A clone was selected to form neurospheres via Embryoid Body from iPS cells, are activated Nanog gene promoter in cells of less than about 0.01% in secondary neurospheres, nerve damage, and clones are selected is administered to a patient having. [Selection Figure 6