Eucaryotic expressive vector coding human hepatocyte growth factor and the human vascular endothelium growth factor, method for producing it and application

摘要

the subject of the invention is eukariotyczny expressive vector coding for human hepatocyte growth factor, vascular endothelial growth factor and human pires / vegf / hgf of general formula 1.eukariotyczny vector, based on the two cdna fragments, creates plasmid pires containing fragment vegf165 cdna to total length of 1224 pz, comprising sequences from 394 to + 818 according to sequence deposited in the bank of genes under number nm _ 003376, and vegf is limited additional sekwen the restrictive for enzymes hindiii and ecor1,recorded in the sequence 1 and a passage for hgf cdna with a total length of 2232 pz, comprising the sequence from - 15 to + 2205 according to sequences deposited in the bank of genes under number nm _ 000601 and hgf is limited additional sequences for xbai and the restrictive enzymes.in addition, both for vegf165 cdna fragment, and a cdna for hgf represent sequences coding for these genes, containing atg at codon as the signal to begin the process of translation, in addition to vegf165 cdna fragment contains at codon tga and cdna for hgf at codon yeah, which signals termination of protein synthesis.the invention also relates to method of manufacturing niewirusowego plasmid vector pires, containing cdna for vegf165 cdna fragment and for hgf, of the general formula 1.the invention concerns also apply eukariotycznego ekspresyjnego vector encoding human vascular endothelial growth factor and human growth factor hepatocytes, for the manufacture of drug therapy for patients with critical limb ischemia intramuscular lower and cho - with hard - goju0105cymi's wounds.