A PCR sequencing method based on DNA molecular tag technique and DNA incomplete shearing strategy

摘要

Abstract: the present invention provides a method to arrange the PCR (PCR sequencing method), are using the strategy of rule prefix index (primer index) + strategy the machines are perfect for DNA (deoxyribonucleic AC ID) (DNA incomplete shearing strategy) + technical arrangements for second generation (second sequencing technique) technique to determine arrangements for double sides (Pair-End sequencing technique)) it is characterized with high productivityLow cost for specific arrangements for second generation (second generation sequencer), making its length can be determined for PCR product obtained the length of the order of sequencing length) Limited. The arrangement (sEquencer), which can expand the scope of its application.The present invention also provides indicators of starting material (primer indexes) method to determine the order of PCR (PCR sequencing method) above, use this method at determination of gene type genotyping, challenge The analysis of HL DaA.