APPLICATION OF A PCR SEQUENCING METHOD, BASED ON DNA BARCODING TECHNIQUE AND DNA INCOMPLETE SHEARING STRATEGY, IN HLA GENOTYPING

摘要

The invention provides a PCR sequencing method, wherein the combination ofprimer indexes, DNA incomplete shearing strategy and the second generationsequencing technique (Paired-End sequencing technique) can make the length ofPCRproducts that can be sequenced by a sequencer longer than the maximumsequencinglength of the sequencer whilst making full use of the characteristics of thesecondgeneration sequencing technique such as high throughput and low cost, therebygreatly broadening its applicable scope. In addition, the present inventionalsoprovides primer indexes for the PCR sequencing method and the use of themethod ingenotyping, particularly in HLA analysis, and also provides the PCR primersused,particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.