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METHOD FOR DETERMINING THE ENDOGENOUS ANTIOXIDATIVE POTENTIAL OF BEVERAGES BY MEANS OF ESR SPECTROSCOPY

机译:ESR光谱法测定饮料的内源性抗氧化能力

摘要

The invention relates to a method for determining the endogenous antioxidative potential (EAP) in a beverage sample, preferably beer, wine, juice or mixtures thereof while using ESR spectroscopy. To be precise, the invention relates to a method for measuring a secondary radical, preferably a hydroxyethyl radical, which is stabilized by a spin trap reagent, the spin trap reagent preferably being N-tert-Butyl-a-(4-pyridyl)nitron-N-oxide (POBN). The invention also relates to a use of the method for assessing the oxidative stability of a beverage sample, to a use of the spin trap reagent, preferably POBN, for measuring the EAP's, and to a kit for carrying out the method. The invention is characterized in that a method is provided with which the pH value effect, which occurs in prior art methods, and the associated influence upon the generation of radicals in the beverage sample to be measured are evaded.
机译:本发明涉及一种使用ESR光谱法测定饮料样品,优选啤酒,葡萄酒,果汁或其混合物中的内源性抗氧化电位(EAP)的方法。确切地说,本发明涉及一种测量通过自旋捕集剂稳定的仲基,优选羟乙基基的方法,该自旋捕集剂优选为N-叔丁基-α-(4-吡啶基)硝基。 -N-氧化物(POBN)。本发明还涉及用于评估饮料样品的氧化稳定性的方法的用途,涉及自旋阱试剂,优选POBN,用于测量EAP的用途,以及涉及用于实施该方法的试剂盒。本发明的特征在于提供了一种方法,利用该方法可以避免在现有技术方法中出现的pH值效应以及对待测饮料样品中自由基产生的相关影响。

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