METHOD OF QUANTITATIVE EVALUATION histological activity in chronic diffuse liver diseases

摘要

A method of quantitative evaluation of histological activity in chronic diffuse liver diseases including liver biopsies, liver tissue fixation in 10% formalin solution, fill the tissue in paraffin, preparation of tissue sections 3-4 micrometers in thickness, H & E stain, the tissue slices with study microscope, count in absolute values, percentage points and pathological signs of necrosis and degeneration of hepatocytes, portal and periportal inflammatory infiltration, necrosis of hepatocytes of step in, characterized in that the detected parenchymal damage and quantitated its components - hydropic, globular and atomizing steatosis, further comprising determining the signs of small cell dysplasia hepatocytes intralobular inflammatory infiltration, central infiltration, then the absolute values ​​of the obtained morphological data converted into points using the proposed protocol quantifying, after which the results point to evaluate changes in liver biopsies n and the values ​​of 1 to 11 points define chronic hepatitis with minimal activity, with values ​​of from 12 to 20 points - chronic hepatitis weak activity, of 21 to 30 - moderate chronic hepatitis activity from 31 to 47 - chronic hepatitis high activity.