METHOD cryopreservation axillary buds ASPEN PLANTS IN VITRO

摘要

1. A method for cryopreservation of axillary buds in vitro aspen plants, comprising insulation axillary buds, preliminary dewatering them in solutions containing nutrient medium and osmolitiki, kidney transfer in cryovials, storage kidney cryovials in liquid nitrogen, and thawing postkriogennoy kidney regeneration of plants are characterized in that the stepwise change of osmotic used media such as liquid osmoliticheskaya I (WPM supplemented with sucrose (0.2-0.5 M) of glycerol (1.7-2.5 M)) osmoliticheskaya liquid II (WPM , sucrose (0.2-0.5 M), g itserol (2.5-3.5 M), ethylene glycol (1-1.5 M), dimethylsulfoxide (1.5-2 M)) and for freezing liquid (WPM, containing sucrose (0.2-0.5 M ), glycerol (3.5-4 M), ethylene glycol (1.5-2.5 M), dimethylsulfoxide (1.5-2 M)) before quick freezing, without the gradual decrease temperatury.2. A method according to claim 1, characterized in that is suitable for prolonged cryo-storage aspen plants genetically modified with recombinant genes.