5 '(5'-UTR) optimized methods of producing recombinant proteins in an artificial DNA sequence and a plant having a reader function to for the overexpression of recombinant proteins in plants

摘要

an artificial DNA for the leader region of the 5'-UTR, the artificial DNA is effective to increase expression of recombinant proteins in plants, Inr initiator site 5 ' 3' in the direction and includes a Kozak or Kozak uniform consensus sequence, and Inr Inishieta site, between the Kozak or Kozak uniform consensus sequence, are formed by composed of oligonucleotide from each of the two or more copies of the adjacent CAA element each other, multiple poly (CAA) or (CAA) _n region, and the same number as the poly (CAA) region, the oligonucleotide composed of two or more copies of the CT element adjacent to each other is formed, comprises a plurality of poly (CT) or (CT) _m region, at least one, one each for any poly (CAA) region, a poly in the 5 ' 3' direction is upstream of (CT) region and at least one poly (CAA) region, 5 ' 3' is adjacent the poly (CT) region in a direction, wherein the artificial DNA is, a / T rich motif, ATT trinucleotide elements, CTG trinucleotide elements, and homopolymer region, ie array consists of many of the same nucleotide than more, or 4, 3, does not contain.