Overexpression of the recombinant xyloglucanase isp-Xeg/ifrom iPenicillium canescens/iaccelerates growth and rooting of transgenic aspen plants

摘要

Analysis of the properties of transgenic aspen clones with recombinant gene xyloglucanase sp-Xeg from fungi Penicillium canescens showed the presence of complex modifications both in the wood and the phenotype of plants. Biometric analysis revealed an increase in the height of transgenic plants as compared to control plants. Increasing in the height of the shoot of 24.8%, 25% and 26% was observed for lines PtXIVXeg1a, PtXVXeg1a, PtXVXeg1b, respectively. Also there was an increase in the number of internodes in some transgenic clones. For the first time we showed the change in plants rhizogenesis with the recombinant gene xyloglucanase. In 10 of the 25 lines the rooting efficiency in vitro exceeded the control value. The maximum value of the rhizogenesis was fixed for line PtXVXeg1a (2.5 times higher than the control value). The mass of the root system for 6 of the 25 clones in the greenhouse was higher by 20% than the control value. The pentosan content decrease was also detected in all wood samples of transgenic plants. The obtained data of xyloglucanase activity and pentosan content generally correlated with phenotypic modifications.