lentiviral vector particle, pseudotyped lentiviral vector genome in an envelope, lentiviral vector packaging system for producing a pesudotyped lentiviral vector particle, cell, isolated nucleic acid molecule, expression vector, host cell, methods for making a particle of lentiviral vector, and to distribute lentiviral vector particles to invitro cells, and therapeutic or prophylactic vaccine

摘要

Lentiviral vector particles comprising a Sindbis virus E2 glycoprotein variant and a lentiviral vector genome comprising a sequence of interest are provided. A lentiviral vector particle comprising: (a) an envelope comprising a Sindbis virus E2 glycoprotein variant; and (b) a lentiviral vector genome comprising a sequence of interest; wherein the E2 glycoprotein variant facilitates infection of dendritic cells by the lentiviral vector particle, and wherein the E2 glycoprotein variant has reduced binding to heparan sulfate compared to a reference sequence (HR strain).