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mutant of a parental trichoderma strain, and methods for producing a polypeptide and for obtaining a mutant of a parental trichoderma strain
mutant of a parental trichoderma strain, and methods for producing a polypeptide and for obtaining a mutant of a parental trichoderma strain
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机译:木霉亲本菌株的突变体,以及生产多肽和获得木霉亲本突变体的方法
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摘要
mutant of a parental trichoderma strain; and, methods for producing a polypeptide and for obtaining a mutant of a parental trichoderma strain. The present invention relates to mutants of a parental trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first protease gene. aspartic, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, with one or more (several) genes being modified making the mutant strain deficient in the production of one. or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to parental trichoderma strain when grown under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods of producing such mutants.
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