CONTRIBUTING TO ANTIGENIC SEQUENCE IN LISTERIA PARTNERS FOR HYBRIDIZATION, PRESENTING SIGNAL PEPTIDES, AND METHODS FOR THEIR RECEIVING AND APPLICATION

摘要

The present invention provides nucleic acids, expression systems, and vaccine strains that efficiently express and secrete antigens of interest into the cytosol of host cells and cause effective CD4 and CD8 T-cell response by functional binding of signal peptides / secretory chaperones Lis N-terminal partners for hybridization in the translational reading frame with selected recombinantly encoded protein antigens. From these N-terminal hybridization partners, any PEST sequences found in nature in this sequence and / or certain hydrophobic residues are removed (by actual removal, by introducing a mutation, or by using a combination of these approaches).