METHOD FOR OPERATING A REAL-TIME POLYMERASE CHAIN REACTION (PCR) SYSTEM AND A DEVICE FOR OPERATING THE METHOD

摘要

The present invention relates to a method for operating a real-time polymerase chain reaction (PCR) system and a device for operating the method. It is the object of the invention to provide a new method, which allows the manufacture of a small and portable real-time PCR system. The object is met by the method for operating a real-time polymerase chain reaction system comprising a control system for temperature regulation of a sample holder, whereby this regulation is of the closed-loop type, for at least two temperature set values, whereby at a first time the closed-loop temperature regulation is started and at a second time, which is after the given first time, the measurement of the fluorescence intensity and/or luminescence decay time starts, whereby at the second time the components of the control system required for the closed-loop temperature regulation are at least partly used for the fluorescence intensity and/or luminescence decay time measurements, while at the second time the closed-loop temperature regulation is interrupted for the measuring period.