METHOD FOR THE SELECTIVE DEPLETION OF ALLOREACTIVE T LYMPHOCYTES FROM DONOR STEM CELL OR LYMPHOCYTE GRAFTS TO PREVENT GRAFT-VERSUS-HOST DISEASE

摘要

Disclosed herein is a method to specifically remove alloreactive T cells. In one example, the method includes culturing donor cells comprising donor T cells with recipient antigen presenting cells (APCs) to form a mixture of cells under conditions sufficient for recipient APCs to activate donor T cells; contacting the mixture of cells with an effective concentration of adenosine or adenosine-like molecule to decrease or inhibit viability of the activated donor T-cells; and removing the adenosine or adenosine-like molecule from the mixture of cells. The resulting cells can be given to the recipient. This method can be used to prevent, reduce or inhibit graft-versus-host disease (GVHD).