Donor-Derived CD19-Targeted T Cell Infusion Eliminates B Cell Acute Lymphoblastic Leukemia Minimal Residual Disease with No Response to Donor Lymphocytes after Allogeneic Hematopoietic Stem Cell Transplantation

摘要

Leukemia relapse is still the leading cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for B cell acute lymphoblastic leukemia (B-ALL).Relapsed patients with BALL after allo-HSCT have a very short median survival.Minimal residual disease (MRD) is predictive of forthcoming hematological relapse after hematopoietic stem cell transplantation (HSCT);furthermore,eliminating MRD effectively prevents relapse.Donor lymphoblastic infusion (DLI) is the main established approach to treat B-ALL with MRD after allo-HSCT.However,about one-third of patients with MRD are non-responsive to DLI and their prognosis worsens.Although donor-derived cluster of differentiation (CD)19-directed chimeric antigen receptor-modified (CAR) T cells (CART19s) can potentially cure leukemia,the efficiency and safety of infusions with these cells have not yet been investigated in patients with MRD after HSCT.Between September 2014 and February 2018,six patients each received one or more infusions of CART19s from HSCT donors.Five (83.33％) achieved MRD-negative remission,and one case was not responsive to the administration of CAR T cells.Three of the six patients are currently alive without leukemia.No patient developed acute graft-versus-host disease (aGVHD),and no patient died of cytokine release syndrome.Donor-derived CAR T cell infusions seem to be an effective and safe intervention for patients with MRD in B-ALL after allo-HSCT and for those who were not responsive to DLI.