Investigation of the callus induction medium and the regeneration medium in rice anther culture.

摘要

Culture medium containing N6 basal medium supplemented with 4 mg 2,4-D/litre effectively induced calli from microspores of some japonica varieties and two F1 japonica hybrids. Regenerated plants containing green and/or albino shoots were produced efficiently from microspore-derived calli on regeneration medium containing LS basal medium supplemented with sorbitol (30000 mg/litre), casein hydrolysate (2000 mg/litre), MES (1000 mg/litre), NAA (2 mg/litre) and kinetin (0.5 mg/litre) solidified by gellun gum (4000 mg/litre).