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METHOD FOR CONSTRUCTING MASS OF MYOCARDIAL CELLS AND USE OF THE MYOCARDIAL CELL MASS

机译:心肌细胞质量的构建方法和心肌细胞质量的使用

摘要

It is an object of the present invention to improve the engraftment rate of purified myocardial cells so as not to include cells other than myocardial cells but to include components derived from other species. In order to solve the above problems, the inventors of the present invention examined whether purified myocardial cells can build up a cell mass. As a result, the purified pluripotent stem cell-derived myocardial cells obtained by dispersing aggregated cell masses containing differentiated and induced myocardial cells from pluripotent stem cells into single cells were cultured in a serum-free medium, The present inventors have made it clear that the above problems can be solved by providing a method for producing cell masses of cardiomyocytes derived from pluripotent stem cells.
机译:本发明的目的是提高纯化的心肌细胞的植入率,以使其不包括除心肌细胞以外的细胞,而不包括源自其他物种的成分。为了解决上述问题,本发明的发明人研究了纯化的心肌细胞是否可以建立细胞团。结果,通过将包含来自多能干细胞的分化的和诱导的心肌细胞的聚集细胞团分散到单个细胞中而获得的纯化的多能干细胞来源的心肌细胞在无血清培养基中培养。本发明人已经清楚了通过提供一种产生多能干细胞的心肌细胞的细胞团的方法,可以解决上述问题。

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