Coupling of the proteins of interest (poi) with viral vectors by means of intein - switched protein splicing

摘要

The present invention provides viruses and viral vectors, as well as a method for the coupling of the proteins of interest in the viruses or viral vectors by means of intein - switched protein ready splicing. The present invention thus enables the covalent coupling of an arbitrary separately produced protein of interest under physiological conditions on the surface of a virus or viral vector after the virus or the viral vector was produced and aufgereiningt. The present invention thus makes it possible, for example, the targeting of viruses or viral vectors for the selective gene transfer and / or the selective infection / transduction of the target cell populations with any one of the transgene. In addition, the described here, viruses or viral vectors be used for the protein transfer. In addition to targeting of viruses or viral vectors, the present invention provides, in addition, possibilities for the coupling of any desired antigens to the surface of viral vectors for the production of potential vaccine vectors.