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METHOD OF DETECTING SIGNAL FROM BACTERIA IN FOOD USING CULTURE COMBINED IN SITU HYBRIDIZATION METHOD

机译:文化结合原位杂交法从食品中细菌中检测信号的方法

摘要

PROBLEM TO BE SOLVED: To address the problem that although livestock products and aquatic products have relatively low fluorescent noise, agricultural products, especially green and yellow vegetables have a significant problem with fluorescent noise due to the inclusion of pigments, a novel automatic detection method of fluorescent signals has been developed which does not generate noise in an even wider range of foods.;SOLUTION: A novel microbial test method which automatically detects fluorescent signals and does not generate noise by detection, by means of detecting a specific color and brightness of a DNA probe-derived fluorescent signal on a membrane filter using a low magnification optical system on proliferating bacteria, using a culture combined in situ hybridization method.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2017,JPO&INPIT
机译:要解决的问题:为了解决以下问题:尽管畜产品和水产品的荧光噪声相对较低,但是农产品(尤其是绿色和黄色蔬菜)由于包含色素而存在荧光噪声的严重问题,这是一种新颖的自动检测方法已经开发出在多种食品中都不会产生噪音的荧光信号。;解决方案:一种新颖的微生物检测方法,可以通过检测食品的特定颜色和亮度自动检测荧光信号,并且不会通过检测而产生噪音。使用组合培养原位杂交方法,通过低倍光学系统对增殖细菌进行膜滤膜上的DNA探针衍生荧光信号。;部分附图:图1;版权:(C)2017,JPO&INPIT

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