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Compositions and methods for identifying replica sequencing reads
Compositions and methods for identifying replica sequencing reads
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机译:用于鉴定复制品测序读段的成分和方法
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摘要
The present invention provides methods, compositions and kits for detecting replica sequencing reads. In some embodiments, the replica sequencing read is removed. In one aspect, a method is provided for detecting a replica sequencing read from a population of sample sequencing leads, the method comprising: a) adapting an adapter to each of a plurality of nucleic acid fragments from one or more samples. Ligating to the 5 ′ end of a nucleic acid fragment, wherein the adapter comprises: (i) an indexing primer binding site; (ii) an indexing site; (iii) an identifier site; and (iv) a target sequence Including a primer binding site; b) amplifying the adapter-nucleic acid fragment ligation product; c) generating a population of sequencing reads from the amplified adapter-nucleic acid fragment ligation product, and the like. Include.
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