SPECIFIC MEDIUM FOR LONG-TERM MAINTENANCE AND PROLIFERATION SUBCULTURE OF HUMAN HEPATOCYTES AND CULTURE METHOD

摘要

A specific culture medium and culture method are for long-term maintenance, proliferation and passaging of human hepatocytes. The culture medium contains a hepatocyte basic medium, a GSK-3 beta inhibitor which is CHIR99021 at a concentration of 0.5-10 μM, a TGF beta inhibitor which is SB 431542 at a concentration of 0.5-10 μM and/or a TGF beta inhibitor which is A83-01 at a concentration of 0.5-10 μM.