expression construct, polynucleotide, cloning vector or expression vector, host cell, method for making a polypeptide, method for producing a bispecific antibody, method for optimizing the expression level of a protein of interest, and method for optimizing the hetero level -dimerization of a protein of interest

摘要

The invention relates to an expression construct for the expression of polypeptides in host cells using alternative splicing. The expression construct can be used for the expression of polypeptides such as antibodies, antibody fragments and bispecific antibodies by expressing the gene products required for protein expression at the ratio leading to the highest titres or the best product quality profile.