A PROCESS FOR DIFFERENTIAL SERO-DIAGNOSIS OF GRAM-NEGATIVE BACTERIAL INFECTIONS AND DETECTION OF THEIR TOXINS IN HUMAN FOOD IN HUMAN FOOD IN REFERENCE TO FISH AND FISH PRODUCTS USING POLYCLONAL ANTIBODIES TO THEIR LESS CROSS REACTING AND BIGHLY SPECIFIC EXTRA CELLULAR PRODUCTS ANTIGEN

摘要

Antigenic sharing among the most commonly bacterial pathogens such as Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescence of Indian major carps has been studied using immunological reactions such as cross agglutination, disc diffusion and indirect enzyme linked immunosorbent assay (ELISA). The data were analysed using statistical analysis (SAS), version 6.12. The result showed high antigenic similarities among the bacterial whole cells, whole cell lysates, somatic "O" antigens, lipo-polysaccharides (LPS) and extra cellular products (ECP). However, few or no similarities were observed in a ECP component. The component was found to be highly specific and less cross-reactive. The same component can be used for the differential diagnosis of bacterial infection and detection of their toxin in the human food. In the process we have developed an antigen capture indirect ELISA to detect the specific bacterial toxin and its components in the fish and fish product and other processed human food.