Composition comprising parasite eggs and methods for isolation and storage of parasite eggs

摘要

A method for isolation and storage of embryonated eggs of the Trichuris suis pig trichocephalus, said method comprises the steps of: a) infecting an animal with Trichuris suis; b) isolate Trichuris suis eggs from the animal's feces after 7-9 weeks, in which the isolation of the eggs comprises a repeated washing procedure on certified steel sieves with reduced mesh size, which includes filtration on a sieve of 50 μm mesh size certified steel followed by filtration on a planned planned 20 μm mesh size sieve, in which the Trichuris suis eggs are isolated in a sieve fraction with a particle size of 20-50 μm; c) resuspend the screened fraction of 20-50 μm with eggs isolated in sulfuric acid, H2SO4, which has a pH value of 0-2; d) clean the isolated egg suspension containing particles in the size range of 20-50 μm by filtration in a disposable nylon net with a certified mesh size of 30-35 μm and recover the eggs on a nylon net of 20 -25 μm; e) repeatedly washing the clean egg suspension in sulfuric acid, H2SO4 having a pH of 0-2, to reduce any pathogen or pathogen spores by dilution; f) embry Trichuris suis eggs by storing the suspension or composition of washed eggs and sulfuric acid, H2SO4 with a pH of 0-2 in an incubator at temperatures of 15-30 ° C in 2 6 months for egg development from non-embryonated eggs until embryonated eggs; and g) store isolated and embryonated eggs at temperatures in the range of 1-10 ° C in sulfuric acid, H2SO4, which has a pH of 0-2, thus allowing the maintenance of biotic potential and the prevention of pathogen growth. .