首页> 外国专利> 7-O- 7-O- Method for Preparing 7-O-Methyl Genistein and 7-O-Methyl Daidzein Using Biotransformation of Genistein and Daidzein

7-O- 7-O- Method for Preparing 7-O-Methyl Genistein and 7-O-Methyl Daidzein Using Biotransformation of Genistein and Daidzein

机译:利用染料木黄酮和大豆苷元的生物转化制备7-O-甲基染料木黄酮和7-O-甲基大豆苷元的7-O- 7-O-方法

摘要

The present invention relates to 7-O-Methyl Genistein or 7-O-Methyl Genistein through biotransformation of Genistein or Daidzein. relates to a method of producing Daidzein), more particularly, a flavonoid of the 7-hydroxyl group (7-hydroxyl group) with O to forward methyl (methyl) - SaOMT2 gene and wherein the methyl transferase encoding the enzyme (O-methyltransferase) A SAM synthetase gene that synthesizes S-adenosine-L-methionine, which is a cofactor of the enzyme, is introduced from genistein or daidzein into 7-O -Methylzynistaine or 7-O-methylididadee. / RTI According to the invention, the 7-hydroxyl group (7-hydroxyl group) with O to forward methyl (methyl) of a flavonoid - SaOMT2 gene and a cofactor of the enzyme adenosine design S- methyl transferase encoding the enzyme (O-methyltransferase) (SAM synthetase), which synthesizes L-methionine (S-adenosine-L-methionine) from a genistein or daidzein using a microorganism variant into which 7- 7-O-Methyl Genistein or 7-O-Methyl Daidzein can be prepared by a conventional method.
机译:本发明涉及通过染料木黄酮或大豆黄酮的生物转化的7-O-甲基染料木黄酮或7-O-甲基染料木黄酮。本发明涉及黄豆苷元的生产方法,更具体地涉及具有 O 以转发甲基(甲基)-SaOMT2基因的7-羟基(7-羟基)的类黄酮,其中甲基转移酶编码酶(O-甲基转移酶)从金雀异黄素或黄豆苷元将合成作为该酶辅因子的S-腺苷-L-蛋氨酸的SAM合成酶基因导入7-O-甲基zy啶或7-O-甲基碘。根据本发明,具有-I的7-羟基(7-羟基)使类黄酮-SaOMT2基因的甲基(甲基)和腺苷酶设计的辅因子S -编码酶(O-甲基转移酶)(SAM合成酶)的甲基转移酶,其使用7-7-O-甲基金雀异黄酮或7-O-甲基大豆苷元可以通过常规方法制备。

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