REASSORTANT STRAIN OF RN2/66-HUMAN A (H7N2) INFLUENZA VIRUS FOR NEURAMINIDASE ANTIBODIES IDENTIFICATION IN CASE OF INFLUENZA INFECTION AND VACCINATION

摘要

FIELD: biotechnology.;SUBSTANCE: diagnostic strain of RN2/66-human A (H7N2) influenza virus was obtained by crossing the equine influenza A/horse/Prague/1/1956(H7N7) virus with the cold-adapted vaccine strain A/17/California/66/395(H2N2) based on the attenuation donor A/Leningrad/134/17/57(H2N2). The strain contains the neuraminidase of the influenza virus subtype N2 A/California/1/1966 (H2N2) and influenza A/horse/Prague/1/1956 (H7N7) hemagglutinin. The RN2/66-human A(H7N2) strain actively multiplies in developing chick embryos at the optimum temperature of 33°C, which allows to accumulate viral material for subsequent purification and concentration. The RN2/66-human A (H7N2) strain can be used for solid-phase inhibition of neuraminidase activity to analyse background values of serum antibodies to influenza neuraminidase N2 in the human population and their changes as a result of infection/vaccination.;EFFECT: improved strain properties.;2 dwg, 1 tbl, 2 ex