modified post-transcriptional regulatory elements of hepatitis

摘要

polynucleotides containing a pre modified are provided having a variant x gene that includes one or more stop codons not present in an unmodified hepatitis x gene such as wild type. Also provided are polynucleotides containing a pre-modified having a variant x gene that includes one or more degradation sequences not present in an unmodified hepatitis x gene, such as wild type. The pre-modified may be operably linked to a nucleic acid encoding a recombinant protein. Expression cassettes, viral vectors and cells containing the polynucleotides, and compositions and methods of use thereof are also provided.