stem cells obtained from deciduous pulp or from permanent teeth and dental germs capable of producing human bone tissue

摘要

stem cells obtained from deciduous pulp or permanent teeth and dental germs, capable of producing human bone tissue. The present invention relates to a method for isolating a new subpopulation of dental pulp-derived stem cells, whose differentiation into osteoblasts leads to the subsequent production and use of bone tissue, called lab (living autologous bone). living autologous bone). specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and dental germs obtained from human patients; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular subpopulation, which, after differentiation into osteoblasts, is capable of producing an in vitro extracellular matrix, identical to that detectable in bone tissue; 3) the application of this selected and differentiated cell population to produce autologous bone tissue in vitro containing vital osteoblasts; 4) preservation of living autologous bone under conditions which guarantee cellular vitality; 5) the application of living autologous bone in donor patients to reconstruct bone tissue, as required in daily dental practice, maxillofacial surgery and orthopedics.