A METHOD OF IDENTIFYING A CELL WITH INCREASED CONCENTRATION OF A PARTICULAR METABOLITE COMPARED TO THE CORRESPONDING WILDTYPE CELL, WHEREIN THE CELL IS MODIFIED VIA RECOMBINEERING, A METHOD FOR THE PRODUCTION OF A GENETICALLY MODIFIED CELL WITH OPTIMISED PRODUCTION OF A PARTICULAR METABOLITE, A METHOD FOR THE PRODUCTION OF SAID METABOLITE AND SUITABLE NUCEIC ACIDS.

摘要

A method for identifying a cell having an intracellular concentration of a particular metabolite that is increased compared to the wild type of the cell, wherein the modification of the cell is achieved by recombineering, and to a method for producing a production cell that is genetically modified compared to the wild type of the cell and has optimized production of a particular metabolite, to a method for producing this metabolite, and to nucleic acids suited therefor. A gene coding for a recombinase, which is homologous to a known recombinase gene, is transformed in a cell using a vector, and a DNA containing at least one modified gene G1 to Gn, or at least one mutation M1 to Mn, is inserted into the cell, and the cell that has highest metabolite production is identified by way of metabolite sensors. A mutation, which is considered to the cause for the increased production, is isolated from this cell, and the gene or the mutation is removed and inserted into a production strain, which thereby exhibits increased production of the metabolite.