METHOD OF IN VITRO DIFFERENTIATION OF NEURAL STEM CELLS, MOTOR NEURONS AND DOPAMINE NEURONS FROM PRIMATE EMBRYONIC STEM CELLS

摘要

A method of differentiating embryonic stem cells into neural and motor cellsis disclosed. In one embodiment, the invention comprises culturing apopulation of cells comprising a majority of cells that are characterized byan early rosette morphology and are Sox1-/Pax6+ in the presence of FGF2, FGF4,FGF8, FGF9, or RA wherein the cells are characterized by an neural tube-likerosette morphology and are Pax6+/Sox1+.