首页> 外国专利> 1 dsRNA Composition for controlling Spodoptera exigua comprising E. coli expressing dsRNA specific to integrin beta 1 subunit of Spodoptera exigua and method for controlling the larvae of Spodoptera exigua using it

1 dsRNA Composition for controlling Spodoptera exigua comprising E. coli expressing dsRNA specific to integrin beta 1 subunit of Spodoptera exigua and method for controlling the larvae of Spodoptera exigua using it

机译:1种用于控制斜纹夜蛾幼虫的双链RNA组合物,其包含表达对斜纹夜蛾整联蛋白β1亚基特异的dsRNA的大肠杆菌,以及使用该组合物控制斜纹夜蛾幼虫的方法。

摘要

The present invention relates to a composition for controlling parasitic worms using E. coli expressing a dsRNA (SEQ ID NO: 1) specific to the integrin 1 subunit (SeINT) of Paralichthys olivaceus, and a method for controlling parasitic wasp. In the present invention, there is provided a composition for controlling parasitic astringency caused by feeding, which comprises a transgenic Escherichia coli expressing a dsRNA (SEQ ID NO: 1) specific to the integrin 1 subunit (SeINT) do. The method for controlling parasitic fever of the present invention comprises the steps of: pre-treating the above-mentioned transformed E. coli with ultrasonic treatment; Feeding the pre-treated transgenic E. coli to the larvae of Paralichthys olivaceus; And treating the Bt toxin after ingestion treatment. According to the present invention, the dsRNA expressed by Escherichia coli can delay the development of the parasitic larva and lead to death, thereby effectively controlling parasitic nematodes.;
机译:本发明涉及一种用于控制寄生虫的组合物和一种用于控制寄生虫的方法,所述大肠杆菌表达特异于寄生虫的整联蛋白1亚基(SeINT)的dsRNA(SEQ ID NO:1)。在本发明中,提供了用于控制由进食引起的寄生性涩味的组合物,其包含表达对整联蛋白1亚基(SeINT)具有特异性的dsRNA(SEQ ID NO:1)的转基因大肠杆菌。本发明的控制寄生虫热的方法包括以下步骤:用超声波处理对上述转化的大肠杆菌进行预处理。将经过预处理的转基因大肠杆菌饲喂到橄榄寄生幼虫的幼虫中;并在摄取治疗后治疗Bt毒素。根据本发明,由大肠杆菌表达的dsRNA可延迟寄生幼虫的发育并导致死亡,从而有效地控制寄生线虫。

著录项

  • 公开/公告号KR101842337B1

    专利类型

  • 公开/公告日2018-03-28

    原文格式PDF

  • 申请/专利权人 안동대학교 산학협력단;

    申请/专利号KR20160024103

  • 发明设计人 김용균;김은성;

    申请日2016-02-29

  • 分类号A01N63/00;C12N1/06;C12N15/70;

  • 国家 KR

  • 入库时间 2022-08-21 12:38:06

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