Enhanced Sleeping Beauty transposon, and metastasis kit and method

摘要

The present invention relates to enhanced Sleeping Beauty (SB) type transposons and methods of metastasis. In particular, the present invention relates to a polynucleotide comprising a cargo nucleic acid flanked by IR / DR on the left and right sides, the IR / DR having a specific sequence is recognized by the SB transposase protein, and the polynucleotide is contained in the cellular DNA. Can be incorporated into. In addition, the present invention lacks a cofactor of metastasis capable of deleting the polynucleotide and an element of the FACT complex, or an inhibitor of cathepsin selected from the group comprising H, S, V and L, or HSP90 Or a cofactor that can be inhibited, a factor that suspends cells in the cell cycle of G0 / G1, G1 / S, or G2 / M, a factor that inhibits PCNA ubiquitination, etc., or these elements are knocked down or inhibited And a kit for transferring a nucleic acid containing additional elements such as cells or cells suspended in the cell cycle. [Selection] Figure 1