Method of whole-genome sequencing

摘要

A whole-genome sequencing method, including: 1) extracting a whole-genome DNA and constructing a BAC library; 2) constructing mixing pools; 3) extracting DNA of the mixing pools; 4) performing pair-end sequencing for the DNA from the mixing pools; 5) scanning sequences of each mixing pool to obtain a feature sequence set and a k-mer set of each mixing pool; 6) analyzing a corresponding feature sequence set and a corresponding k-mer set of each clone; 7) constructing clone contigs; 8) dividing the k-mer sets of the clones into small k-mer sets and positioning the small k-mer sets; 9) assembling the NGS sequences to yield sequence contigs; 10) positioning the sequence contigs, connecting the sequence contigs and determining directions thereof; and 11) determining relative positions and directions of the clone contigs, and connecting the sequences of clone contigs as whole stripe of chromosome sequences.