首页>
外国专利>
Systems and methods for the measurement of tacrolimus in oral fluids by liquid chromatography tandem mass spectrometry
Systems and methods for the measurement of tacrolimus in oral fluids by liquid chromatography tandem mass spectrometry
展开▼
机译:液相色谱串联质谱法测定口腔液中他克莫司的系统和方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
Disclosed is a method of measuring tacrolimus levels in a subject. In exemplary embodiments, the method comprises the steps of: collecting oral fluid from the subject; homogenizing the oral fluid; combining the homogenized oral fluid with a precipitating solvent; separating oral fluid components on a liquid chromatography column by gradient elution with a mixture of a solvent A and a solvent B, wherein the solvent A is about 2 mM ammonium acetate/0.1% (v/v) formic acid in water and solvent B is about 2 mM ammonium acetate/0.1% (v/v) formic acid in MeOH and wherein the amount of solvent B is increased from about 50% (v/v) to about 98% (v/v); and quantifying the amount of tacrolimus in the oral fluid by mass spectrometry.
展开▼